Effects of CD147 on the regulation of MDR1, MMP2 and MMP9 gene expression. MCF7, MCF7/pCDNA3.0, MT1, 2, 3 cells and MCF7/Adr, MCF7/Adr/pSUPER, AT1, 2, 3 cells were harvested, total RNA were extracted, and lysates were electrophoresed through 8% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Endogenous MDR1, MMP2 and MMP9 mRNA (a, c) and protein levels (b, d) were measured by real‐time polymerase chain reaction (PCR) and Western blot, respectively. Bar graphs represent mean ± SEM of three independent experiments. **P < 0.05 cs control cells.