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. 2009 Jan 13;100(4):639–645. doi: 10.1111/j.1349-7006.2008.01078.x

Figure 3.

Figure 3

Short interfering RNA (siRNA)‐mediated knockdown of endogenous chicken ovalbumin upstream promoter transcription factor (COUP‐TF) II protein in MCF‐7 cells. Knockdown cells were subjected to immunoblotting and reverse transcription–polymerase chain reaction analysis 3 days after transfection of siRNA. (a) COUP‐TFII immunoreactivity was detected in the protein extracts of cells transfected with non‐specific siRNA (lane 1). COUP‐TFII immunoreactivity was decreased in the cells treated with COUP‐TFII‐specific siRNA (lanes 2 and 3). Vascular endothelial growth factor (VEGF)‐C mRNA expression was also repressed in the cells treated with COUP‐TFII‐specific siRNA compared to cells treated with non‐specific siRNA in the reverse transcription–polymerase chain reaction analysis.