Table 1.

 PCR primers of either cell‐cycle‐ or apoptosis‐associated genes

Genes	Primers (5′‐3′)		Product size (bp)
	Sense	Anti‐sense
G2/M cell cycle
Cyclin A	gctatcctcgtggactggtt	cagcaataactgatggcaaatac	436
Cyclin B1	gtcaccaggaactcgaaaa	tccagtgacttcccgacc	585
Cyclin B2	acggtgtccagtgatttg	atcttcgttatcaatgtcctc	351
Cyclin H	agccagcaatgccaagat	cagaatgacatcgctccaact	633
p21	ggacctgtcactgtcttgtacc	ttcctgtgggcggattag	177
CDK1 (CDC2)	ccctcctggtcagtacatg	gctaggcttcctggtttcc	468
CDK7	tgtgggctgttggctgta	ttcttgggcaatcctcct	442
Apoptosis
Death receptor pathway
Fas	ttctgccataagccctgtc	ttggtgttgctggtgagtg	320
FasL	tcttccctgtccaacctct	tgagttggacttgcctgtt	379
TNF‐α	ctccaggcggtgcttgttc	tggtaggagacggcgatgc	414
TRAIL	aagtggcattgcttgtttct	ttgatgattcccaggagttta	320
TRAILR1 (DR4)	ttgttgcatcggctcaggtt	gaggcgttccgtccagtttt	489
TRAILR2 (DR5)	acccttgtgctcgttgtcgc	tgggctggaggtcaccgtct	498
DR6	gggaccaaggagacagacaacg	ccacaatcaccacaagcaccag	493
Bcl‐2 family
Anti‐apoptosis
Bcl‐2	tcgccctgtggatgactgag	cagagtcttcagagacagccagga	143
Bcl‐Xl	gggactgaatcggagatggaga	gctggacggaggatgtggtg	650
MCL‐1 isoform 1	cagcgacggcgtaacaaa	ccaccctaccatcttcactaaatc	584
Pro‐apoptosis
Bax	tcaggatgcgtccaccaa	gttgaagttgccgtcagaaa	166
Bcl‐Xs	gggactgaatcggagatggaga	gctggacggaggatgtggtg	551
Bad	tcccagagtttgagccga	ccaagttccgatcccacc	468
Bak	ggacgacatcaaccgacgctat	tctgaaccgggacccaaagg	424
Bid	cagtgggagggctacgat	taggtgcgtaggttctggtta	407
Bik	ctggaccctatggaggactt	cagtctggtcgtagatgaaagc	178
Bim	ccctacagacagagccaca	attcgtgggtggtcttcg	439
MCL‐1 isoform 2	ccgcttgaggagatggaa	ccaccctaccatcttcactaaatc	781
Internal control
β‐Actin	cccagagcaagagaggcatc	catgatctgggtcatcttctcg	197

CDK, cyclin‐dependent kinase; DR, death receptor; TNF‐α, Tumor necrosis factor‐α; TRAILR, TNF‐related apoptosis inducing receptor.