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. 2010 Nov 15;102(1):206–211. doi: 10.1111/j.1349-7006.2010.01772.x

Figure 4.

Figure 4

 Effect of corosolic acid on signal transducer and activator of transcription‐3 (STAT3) and nuclear factor‐kappa B (NF‐κB) activation in human monocyte‐derived macrophages (HMDM) and glioblastoma cells. (A) The THP‐1 macrophages were incubated with the indicated concentration of corosolic acid for 1 h after treatment with tumor culture supernatants (TCS) for 24 h, followed by determination of phosphorylated STAT3 expression by immunohistochemistry, as described in the Materials and Methods. (B) The number of macrophages positive for phosphorylated STAT3. (C) The HMDM were incubated with corosolic acid (30 μM) for 1 h after treatment with IL‐10 (20 nM) or TCS for 24 h, followed by the determination of phosphorylated STAT3, STAT3, phosphorylated JAK, phosphorylated NF‐κB, β‐actin and lamin expression by western blot analysis, as described in the Materials and Methods. (D) The U373 cells were incubated with the indicated concentrations of corosolic acid for 1 h, followed by determination of phosphorylated STAT3, STAT3, phosphorylated NF‐κB, β‐actin and lamin expression by western blot analysis, as described in the Materials and Methods.