Figure 1.
Immunohistochemical analysis of CD68‐ and CD163‐positive cells in intrahepatic cholangiocarcinoma. (a) In patient no. 3 (Pt.3), CD68‐positive tumor‐associated macrophages (TAMs) highly expressed CD163. However, CD163 was not detected in CD68‐positive TAMs in patient no. 25 (Pt.25). The density of macrophages was classified into two groups (high density or low density). (b) Double‐immunostaining of CD68 and CD163 antigens were performed. CD68 (brown) and CD163 (blue) antigens were mainly localized in cytoplasm and surface cell membrane respectively. Double‐positive cells are indicated by arrows.