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. 2009 Oct 10;101(1):201–209. doi: 10.1111/j.1349-7006.2009.01392.x

Figure 4.

Figure 4

 Quantification of C1q molecules bound to immunocomplexes. (a) RC‐K8 cells were incubated with humanized anti‐CD20 monoclonal antibodies (mAbs) at various concentrations for 15 min, followed by the addition of normal human serum to 1% v/v. Relative amounts of bound C1q molecules were determined using fluorescein isothiocyanate (FITC)‐conjugated anti‐C1q polyclonal antibodies (Abs). Data points represent mean values ± SD of three samples. (b) RC‐K8 cells were incubated with 5 μg/mL humanized anti‐CD20 mAbs for 15 min. Relative amounts of bound humanized anti‐CD20 mAbs were determined using FITC‐conjugated secondary Abs. Data points represent mean values ± SD of three samples. Ab, antibody concentration.