Figure 2.
Expression of microRNAs (miRNAs)‐143 and ‐145 and cell growth in B‐cell cultured cell lines. (a) Evaluation of expression of miRNAs‐143 and ‐145 in human Epstein‐Barr virus (EBV)‐transformed B‐cell lines and Burkitt lymphoma cell lines by use of semi‐quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) and TaqMan MicroRNA assays using real‐time PCR. U6 was used as an internal standard. CD19+ B‐cell‐1 was designated as 1 in quantitative RT‐PCR by TaqMan miRNA assays using real‐time PCR. The results of real‐time PCR were expressed as the mean values of two independent experiments. (b) Steady‐state cell growth of EBV‐transformed B‐cell lines at 72 h after seeding at the concentration of 1 × 105/mL (c) Western blot analysis of ERK5 and c‐myc in control and B‐cell lines in the same samples as in (b), and in human Burkitt lymphoma cell lines. β‐actin was used as an internal control.