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. 2008 Oct 14;99(10):1992–2003. doi: 10.1111/j.1349-7006.2008.00955.x

Figure 2.

Figure 2

Activated Akt/mammalian target of rapamycin (mTOR) signaling pathway is present in resistant MCF‐7‐derived breast cancer (R‐MVLN) cells. Western blot analysis of (a) phospho‐ser473 Akt and total Akt, (b) phospho‐ser167 estrogen receptor alpha (ERα) and total ERα, (c) phospho‐ser136 Bad, (d) phospho‐ser21/9 GSK3α/β, (e) IGF1Rα and (f) phospho‐thr389 p70S6 K and total p70S6 K as described in the Materials and methods section using specific antibodies. Expression of α‐tubulin was measured as an invariant control. The Western blots shown are from one representative experiment that was reproduced at least three times in independent experiments and cell lysates. Histograms represent means ± SD of protein expression levels in independent experiments (*P < 0.05, **P < 0.01 and ***P < 0.001 according to Student's t‐test).