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. 2007 Jul 19;98(9):1330–1335. doi: 10.1111/j.1349-7006.2007.00547.x

Figure 5.

Figure 5

Growth differentiation factor 15 (GDF15)‐induced GDF15 mRNA synthesis. MCF‐7 cells in 35‐mm diameter dishes were transfected with control or an membrane type (MT)1‐matrix metalloproteinase (MMP) plasmid (2 g), and were incubated with or without a recombinant GDF15 protein (25 ng/mL) for 9 h. A total mRNA was extracted, and was subjected to real‐time polymerase chain reaction assay to compare the GDF15 mRNA level as described in Materials and Methods. The highest mRNA level was arbitrarily set to 100%, and the mRNA levels of other cells were adjusted accordingly.