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. 2007 Jul 19;98(9):1330–1335. doi: 10.1111/j.1349-7006.2007.00547.x

Figure 6.

Figure 6

Effect of membrane type (MT)1‐matrix metalloproteinase (MMP) expression on growth differentiation factor 15 (GDF15)‐induced cell‐growth suppression. (a) control or a recombinant GDF15 (25 ng) was added to the 1‐mL culture of MCF7 cells transfected with a control plasmid or MT1‐MMP plasmid with or without BB94 and the cell number was compared after 24 h as described in Materials and Methods. The highest cell number was arbitrarily set to 100%, and the numbers of other cells were adjusted accordingly. Bars indicate mean value. *P < 0.001. (b) HT1080 cells stably transfected with the GDF15‐FLAG plasmid were incubated for 24 h in the presence or absence of BB94 or Concanavalin A (10 g/mL). Conditioned medium proteins were separated on Tris‐Tricine sodium dodecyl sulfate–polyacrylamide gel electrophoresis and blotted using the FLAG M2 antibody. Note that the level of GDF15 mature form (12.5‐kDa) increased by BB94 treatment. (c) HT1080 cells stably transfected with control plasmid or GDF15‐FLAG plasmid were cultured in the presence or absence of BB94 for 24 h, and the cell number was compared as described above. *P < 0.001.