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. 2009 Feb 24;100(5):948–955. doi: 10.1111/j.1349-7006.2009.01111.x

Figure 1.

Figure 1

The mRNA expression of Ihh and receptor molecules. The expression of Indian hedgehog (Ihh), Patched (Ptc), Smoothened (Smo), glioma‐associated oncogene homolog (GLI)1 and GLI2 mRNA in leukemic cell lines and primary CD34+ leukemic cells was analyzed by reverse transcription – polymerase chain reaction. The expression of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was used as an internal standard. Lane 1, NB4; Lane 2, U937; Lane 3, HL60; Lane 4, KG1; Lane 5, Kasumi‐1; Lane 6, Kasumi‐3; Lane 7, TF‐1; Lane 8–10, primary CD34+ leukemic cells derived from three acute myeloid leukemia (AML) patients; Lane 11, bone marrow (BM) CD34+ cells; Lane 12, H2O.