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. 2008 Dec 22;100(3):441–448. doi: 10.1111/j.1349-7006.2008.01067.x

Figure 1.

Figure 1

Expression of p120‐catenin (p120ctn) after transfection of p120ctn‐small invasive (si)RNA.Following transfection with siRNAs as described in the Materials and Methods section, p120ctn expression was analyzed by Western blot and reverse transcription – polymerase reaction (RT‐PCR). To test the specificity of our siRNAs, we re‐introduced p120ctn cDNA to three clonal cell lines. (A) Protein bands representing p120ctn isoforms were detected at approximately 120 kDa (isoform 1) and 100 kDa (isoform 3) in BE1 and control cells. The clonal cell lines designated siRNA1‐3 revealed more than 90% reduction in p120ctn. (B) RT‐PCR analysis indicates that mRNA expression of p120ctn produced several specific bands, in BE1 and control cells. After transfection with p120ctn‐siRNA, the BE1 cells showed a reduction or the complete absence of all p120ctn isoforms. (C) Lanes 1–3 were siRNA‐transfected cells transfected with mouse p120ctn 1A, and lanes 4–6 were the siRNA‐transfected cells transfected with p120ctn 3A. At approximately 120 kDa and 100 kDa, protein bands representing p120ctn isoform 1 or isoform 3, respectively, were detected. SPC (D–F) and LTE (G–I) cells showed similar results.