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. 2008 Aug 14;99(8):1679–1684. doi: 10.1111/j.1349-7006.2008.00857.x

Figure 1.

Figure 1

Autophosphorylation of epidermal growth factor receptor (EGFR) and expression levels of surfactant proteins (SP)‐A, ‐B, ‐C and ‐D in H441 cells. H441 cells cultured in serum‐free RPMI‐1640 medium plus 20 µg/mL EGF were treated with 1 µM gefitinib for 48 h. (a) Western blotting of whole EGFR protein and autophosphorylated EGFR protein in H441 cells after gefitinib treatment. (b) Reverse transcription polymerase chain reaction of SP‐A, ‐B, ‐C and ‐D in H441 cells after gefitinib treatment. (c) Immunohistochemistry of SP‐A on H441 cells after gefitinib treatment. P‐EGFR.