Figure 6.

 Functional analysis of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) knockdown in glioblastoma cells. (a) Stable transfection of U‐87 cells with shPRAF2 significantly reduced PRAF2 protein expression compared to controls (wild type and scrambled). Tubulin served as a loading control. (b) The cell viability was determined at 24, 48, and 72 h in triplicate wells in two separate experiments with similar results. (c,d) PRAF2 down‐regulation inhibits cell migration (c) and invasion (d). Five × 10⁴ cells were seeded in the top chamber of Transwell plates. Serum‐induced migration/invasion was measured after 24 h. PRAF2 down‐regulation appeared to inhibit glioblastoma migration and invasion, compared to wild‐type and scrambled control glioblastoma cells. Three independent experiments were performed in triplicates and data are represented as mean ± SD (n = 9). WT, wild type.