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. 2010 Aug 4;101(11):2341–2350. doi: 10.1111/j.1349-7006.2010.01704.x

Figure 1.

Figure 1

 Expression of plasmid granulocyte macrophage colony stimulating factor (pGM‐CSF) and plasmid macrophage inflammatory protein‐3 alpha (pMIP3α) vectors in vitro. EL4/MUC1 tumor cells were transiently transfected with the pcDNA3.1 (0.4 μg/μL), pGM‐CSF (0.4 μg/μL) and pMIP3α (0.4 μg/μL) vector using the Lipofectamine 2000 (Invitrogen). (a,b) The expression of pGM‐CSF and pMIPα were determined by ELISA. Cell supernatants of EL4/MUC1 tumor cells transfected with pGM‐CSF or pMIPα were evaluated after 2 days. GM‐CSF and MIP3α were detected by ELISA after serially diluting supernatants. A representative result of three experiments is shown. Columns contain mean values. Bars are means ± SD.