Figure 3.

 Immunohistochemistry of tumor sections injected with various vectors (pcDNA3.1, plasmid granulocyte macrophage colony stimulating factor [pGM‐CSF], plasmid macrophage inflammatory protein‐3 alpha [pMIP3α], and pGM‐CSF plus pMIP3α). EL4/MUC1 tumor cells (5 × 10⁴) were administered s.c. to C57BL/6 mice. The various vectors, namely pcDNA3.1, pGM‐CSF, pMIP3α, and pGM‐CSF plus pMIP3α, were injected intratumorally into the EL4/MUC1 tumors on days 10, 12 and 14 after tumor cell inoculation. Frozen tumor sections were stained with anti‐CD11c antibody or isotype IgG as a control (magnification, ×200). No staining was observed using isotype‐matched control antibodies. The result shown is representative of two experiments.