Table 1.

 Immunohistochemical analysis of tumor mass from C57BL/6 mice at day 7 after co‐injection with pGM‐CSF and pMIP3α

Plasmid injected	CD11c (SD)
pcDNA3.1	0.57 (0.53)
pGM‐CSF	1.86 (0.38)
pMIP3a	2.00 (0.58)
pGM‐CSF+ pMIP3α	3.57 (0.53)

Immunohistochemistry of sections of tumors injected with the various vectors (pcDNA3.1, pGM‐CSF, pMIP3α, and pGM‐CSF plus pMIP3α). The various vectors, namely pcDNA3.1, pGM‐CSF, pMIP3α, and pGM‐CSF plus pMIP3α were injected intratumorally into EL4/MUC1 tumors on days 10, 12 and 14 after tumor cell inoculation. Frozen tumor sections were stained with anti‐CD11c Ab or isotype IgG as a control (magnification, ×200). Staining of CD11c+‐expressing cells was graded on a 0 ∼ 4 scale: 0, no positive staining/×200); 1, <9 stained cells/×200); 2, 11∼30 stained cells/×200);3, <30‐50 stained cells/×200); 4, 31∼50 stained cells/×200). Results are given as an average of eight tumor masses). pGM‐CSF, plasmid granulocyte macrophage colony stimulating factor; pMIP3α, plasmid macrophage inflammatory protein‐3 alpha; SD, standard deviation.