Table 2.

 Median survival time (MST) of mice

Treatment	MST (days)
pcDNA3.133.4 ± 2.1
pGM‐CSF 43.5 ± 3.2*
pMIP3a43.2 ± 2.2*
pGM‐CSF + pMIP3a	60.7 ± 4.3**

EL4/MUC1 tumor cells (5×10⁴) were resuspended in 0.1 mL of PBS and inoculated subcutaneously into the right hind legs of mice. pcDNA3.1, pGM‐CSF, pMIP3α, and pGM‐CSF plus pMIP3α vectors (100 μg each) were resuspended in 100 μL of PBS and injected into EL4/MUC1 tumors on days 10, 12 and 14 after tumor cell inoculation. pGM‐CSF plus pMIP3α (**P < 0.01 vs pcDNA3.1; *P < 0.05 vs pGM‐CSF alone; *P < 0.05 vs pMIP3α alone), pGM‐CSF alone (*P < 0.05 vs pcDNA3.1; no significance versus pMIP3α alone) and pMIP3α (*P < 0.05 vs pcDNA3.1). pGM‐CSF, plasmid granulocyte macrophage colony stimulating factor; pMIP3α, plasmid macrophage inflammatory protein‐3 alpha.