Figure 5.

 Importance of endogenous interferon (IFN)‐γ for natural killer (NK) cell MCA205 tumor infiltration and early antitumor immune responses. B6 wild‐type or IFN‐γ^−/− mice were inoculated s.c. with MCA205 fibrosarcoma (4 × 10⁵) and tumors were harvested 1 week after inoculation. Tumor‐infiltrating lymphocytes (TIL) were isolated and then subjected to flow cytometry analysis. The representative FACS plots from the indicated groups of mice are shown and numbers represent the percentage of cells in the different gates (A). The proportion of NK cells (NK1.1⁺ CD3⁻ cells) from the indicated groups of mice are presented (B). The data represent the mean ± SEM and are representative of two experiments. (C) B6 mice or IFN‐γ^−/− mice were inoculated s.c. with MCA205 fibrosarcoma (4 × 10⁵) with or without anti‐NK1.1 treatment. The data are represented as the mean ± SEM on day 7 after tumor inoculation. *P < 0.05 compared with untreated wild‐type (WT) mice. NS, not significant.