Table 2.
Primer sequences and restriction enzymes
| Gene | Polymorphisms | Primers (5′–3′) | Restriction enzyme |
|---|---|---|---|
| EGF | G61A (rs4444903) | Forward: TGTCACTAAAGGAAAGGA Reverse: TTCACAGAGTTTAACAGCCC | AluI |
| TGFA | TaqI (rs11466267) | Forward: TTGTTTTGTTTTTTGAGACGG Reverse: GTGTGAGACTTTTCCAGCCCTGT | TaqI |
| RsaI (rs3732248) | Forward: TGCCTCACCACGACAGACACA Reverse: TGAATAACCCCAAGCAGACGG | RsaI | |
| BamHI (rs11466297) | Forward: ACAGATGGCGGAAGCAGAGGT Reverse: CTAAAGGGCAAGGAAACACAG | BamHI | |
| C3296T (rs2166975) | Forward: GCTCTGCCATCTCCAAGT Reverse: ATCTCTGGCAGTGCTGTCCT | — | |
| C3827T (rs1058213) | Forward: TGGGGAAGAAAGTGAAGGAG Reverse: ATCTCCAAGGGTGGCGATAG | — | |
| TS | 28‐bp repeat in enhancer region (rs45445694) | Forward: GTGGCTCCTGCGTTTCCCCC Reverse: GCTCCGAGCCGGCCACAGGCATGGCGCGG | — |
| G/C SNP in second repeat (rs2853542) | Forward: GTGGCTCCTGCGTTTCCCCC Reverse: GCTCCGAGCCGGCCACAGGCATGGCGCGG | HaeIII | |
| 6‐bp deletion in 3′‐UTR (rs16430) | Forward: CAAATCTGAGGGAGCTGAGT Reverse: CAGATAAGTGGCAGTACAGA | DraI | |
| ERCC1 | Asn118Asn (rs11615) | Forward: TCATCCCTATTGATGGCTTCTGCCC Reverse: GACCATGCCCAGAGGCTTCTCATAG | BsrDI |
| C8092A (rs3212986) | Forward: CAGAGACAGTGCCCCAAGAG Reverse: GGGCACCTTCAGCTTTCTTT | MboII | |
| XPD | Arg156Arg (rs238406) | Forward: CACACCTGGCTCATTTTTGTAT Reverse: TCATCCAGGTTGTAGATGCCA | TfiI |
| Asp312Asn (rs1799793) | Forward: CTGTTGGTGGGTGCCCGTATCTGTTGGTCT Reverse: (TAATA)TCGGGGCTCACCCTGCAGCACTTCCT | StyI | |
| FCGR2A | His166Arg (rs1801274) | Forward: GGAAAATCCCAGAAATTCTCGC Reverse: CAGCGTGTAGCCTATGTTTCC | BstU1 |
| FCGR3A | Val212Phe (rs396991) | Forward: TGGCAAAGGCAGGAAGTATT Reverse: ATTGCAGGTTCCACACACAG | — |
EGF, epidermal growth factor; ERCC1, excision repair cross‐complementation group 1; FCGR, fragment c gamma receptor; SNP, single nucleotide polymorphism; TGFA, transforming growth factor‐α; TS, thymidylate synthase; UTR, untranslated region; XPD, Xeroderma pigmentosum group D.