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. 2007 Dec 27;99(3):524–530. doi: 10.1111/j.1349-7006.2007.00707.x

Figure 3.

Figure 3

Depletion of fragile histidine triad (Fhit) by small interfering RNA (siRNA) downregulates Chk2 phosphorylation in response to ionizing radiation (IR) in HEK293 cells. (A) Knockdown of Fhit expression by transfection of FHIT‐specific siRNA (siFHIT). (B) Depletion of Fhit expression decreased pChk2 levels in response to IR. Forty‐eight hours after siRNA transfection (10 nM), cells were exposed to IR at a different condition: (a) time course (0–12 h) and (b) dose‐dependent (0–10 Gy) analyses were conducted.