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. 2011 Jun 23;102(8):1509–1515. doi: 10.1111/j.1349-7006.2011.01978.x

Figure 2.

Figure 2

 Varying expression levels of thymidylate synthase (TS) protein and the TS–5‐fluoro‐dUMP (FdUMP) complex in the human gastric cancer cell lines before or after 5‐fluorouracil (5‐FU) treatment. MKN‐1, SH‐10‐TC, MKN‐74, MKN‐28, KATO III and MIN‐45 cells were treated with 5 μM 5‐FU for 0 (no treatment), 1 day (24 h) or 2 days (48 h). Extracts of control (0) and 5‐FU‐treated cells were analyzed by Western blotting using anti‐TS antibody (upper panel) or anti‐β‐actin antibody (lower panel). TS* represents the TS‐FdUMP complex.