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Time course of changes in expression levels of thymidylate synthase (TS) and the TS–5‐fluoro‐dUMP (FdUMP) complex (TS*) in 5‐fluorouracil (5‐FU)‐resistant (A‐C) and 5‐FU‐sensitive (D‐F) gastric cancer cell lines after treatment with 5 μM 5‐FU for the periods indicated. The cell extracts were analyzed by Western blotting using anti‐TS, anti‐orotate phosphoribosyltransferase (OPRT) and anti‐β‐actin antibodies.
