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. 2006 Oct 17;98(1):32–36. doi: 10.1111/j.1349-7006.2006.00338.x

Figure 2.

Figure 2

DNA methylation status and expression of RIZ1 in prostate cancer (PCa) cell lines. (A) Methylation‐specific polymerase chain reaction (MSP) of RIZ1. The methylated allele was detected only in the PC3 cell line. U, unmethylated polymerase chain reaction (PCR) product; M, methylated PCR product. (B) Reverse transcription–PCR of mRNA from PCa cell lines. RIZ1 is not expressed in the PC3 cell line. (C) Effect of 5‐Aza‐2′‐deoxycytidine (Aza‐dC) treatment. Aza‐dC‐treated LNCaP and PC3 cells and untreated LNCaP and PC3 cells were analyzed by MSP. The unmethylated allele was detected in Aza‐dC‐treated PC3 cells but not in untreated PC3 cells (D) Expression of RIZ1 was analyzed in Aza‐dC‐treated LNCaP and PC3 cells and untreated LNCaP and PC3 cells. RIZ1 mRNA is expressed in Aza‐dC‐treated PC3 cells but not in untreated PC3 cells.