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. 2008 Apr 14;99(6):1279–1286. doi: 10.1111/j.1349-7006.2008.00791.x

Figure 5.

Figure 5

Detection of soluble major histocompatibility complex class I‐related chain A (sMICA) by enzyme‐linked immunosorbent assay (ELISA) in culture supernatants of Hela and K562 cell lines. Hela and K562 cell lines were treated with 1000 u/mL interferon (IFN)‐γ or medium and in the presence of neutralizing antibody to IFNγ or matrix metalloproteinase (MMP) inhibitor GM6001 for 72 h. Then culture supernatants were collected and used for detection of sMICA by ELISA. Data are mean ± SEM from three independent experiments. **P < 0.01 compared with unstimulated control. ## P < 0.01 compared with the IFNγ‐stimulated group.