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. 2010 Jul 6;101(11):2462–2469. doi: 10.1111/j.1349-7006.2010.01668.x

Figure 2.

Figure 2

 Nobiletin reduced proMMP‐2 levels via downregulation of MMP‐2 mRNA expression in human umbilical vein endothelial cells. (A) The cells were treated with indicated concentrations of nobiletin for 18 h. Gelatinase activity in the conditioned medium was analyzed using a gelatin zymography. The conditioned medium of HT‐1080 cells was used as a marker for MMP‐2 and MMP‐9. (B) MMP‐2 and GAPDH mRNA expressions were analyzed by northern blotting. The ratio of MMP‐2 to GAPDH was calculated after each radioactive band was quantified. All values are expressed as means ± SE (n = 3). *P < 0.05, **P < 0.01, as compared with the control.