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. 2008 Oct 30;100(1):47–53. doi: 10.1111/j.1349-7006.2008.00991.x

Figure 1.

Figure 1

Apoptotic cell death induced by overexpressed papillomavirus binding factor (PBF). (a) LDH release from 293EBNA cells and OS2000 cells transiently transfected with PBF‐myc/pcDNA3.1+ or pcDNA3.1+ as mock transfection. (b) The proportion of cell death in 293EBNA cells transiently transfected with PBF‐myc/pcDNA3.1+ or mock transfection. (c) Expression of PBF and cleaved poly(ADP‐ribose) polymerase (cPARP) in 293EBNA cells was assessed from 0 to 27 h after transient transfection with PBF‐myc/pcDNA3.1+ or mock transfection. (d) Caspase‐3 activity in 293EBNA cells transiently transfected with PBF‐myc/pCMV or pCMV as mock transfection. (e) Expression of PBF, caspase‐9, and cPARP in 293EBNA cells was assessed after transient transfection with the indicated plasmids. IB, immunoblotting.