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. 2011 Jul 27;102(10):1815–1821. doi: 10.1111/j.1349-7006.2011.02024.x

Figure 2.

Figure 2

 Effect of HSulf‐1 on the migration and invasion of cells from the tow gastric cancer cell lines. (a) Wound healing assay. Wound size was monitored (top) and measured (bottom) at the time points indicated in MKN28 and AGS cells stably transfected with either empty vector (Inline graphic) or HSulf‐1 (Inline graphic). Scale bar, 200 μm. (b) Transwell migration assay of MKN28 and AGS cells stably transfected with empty vector or HSulf‐1. OD560, optical density at 560 nm. (c) The invasion assay for MKN28 cells stably transfected with empty vector or HSulf‐1. Data are the mean ± SEM percentage absorbance at 560 nm compared with control. *P < 0.05, **P < 0.01 compared with empty vector. (d) Semiquantitative RT‐PCR analysis of the mRNA expression of metastasis‐related genes in MKN28 and AGS cells stably transfected with empty vector or HSulf‐1.