Table 1.

 Primer sequences, fragment size, annealing temperatures, and number of PCR cycles used for methylation‐specific PCR

	5′ → 3′	Size (bp)	Annealing temperature (°C)	Number of PCR cycles
APC unmethylated	F: AATTTGTTGGATGTGGATTAGGGT R: AACCTCATATCAATCACATACA	89	61	35
APC methylated	F: CGTTGGATGCGGATTAGGGC R: CCTCATATCGATCACGTACG	84	61	35
E‐cadherin unmethylated	F: TAATTTTAGGTTAGAGGGTTATTGT R: CACAACCAATCAACAACACA	97	59	37
E‐cadherin methylated	F: TTAGGTTAGAGGGTTATCGCGT R: TAACTAAAAATTCACCTACCGAC	116	61	37

APC, adenomatous polyposis coli; E‐cadherin, epithelial cadherin; F, forward; R, reverse.