Figure 7.
Knock down of α/β hydrolase domain containing 6 (ABHD6) in Ewing family tumor (EFT) cells did not influence cell growth. ABHD6 was knocked down in the EFT cell line SK‐N‐MC as described in Materials and Methods. Cells transfected with empty vector were used as a control. (a) After selection of stable blasticidin‐resistant cells, expression of ABDH6 was analyzed by RT‐PCR. β‐Actin (ACTB) and lipase I (LIPI) served as controls. (b) The same cells as in (a) were placed at a cell density of 1 × 105/mL in 10 mL culture medium. After 4 days cells were harvested and counted. Bars represent means and standard deviations from three independent experiments. In each experiment cell numbers were determined in quadruplicate. miR ABHD6, cells transfected with ABHD6‐specific RNAi expression vector.