Figure 2.

MUC1 DNA vaccine effectively suppressed tumor growth at orthotopic sites and in the liver metastases in a MUC1‐specific manner. One week after the final MUC1 DNA immunization, SL4‐P36 or SL4‐M11 cells (1 × 10⁶ cells/50 µL) were injected into the space under the cecal serosa of mice. (a) Twenty‐one days after the challenge, the mice were killed and the weight of the cecum was examined. In the liver metastasis model, SL4‐P46 or SL4‐M8 cells (5 × 10⁵ cells/50 µL) were injected into the spleen. Nineteen days after the challenge, the weights of the (b) spleen and (c) liver were measured. Open circles represent tumor‐free mice and filled circles represent tumor‐bearing mice. n.s., not significant; *P < 0.005.