Figure 8.

Tumor cells from the ceca, spleens, and livers were sensitive to tumor necrosis factor (TNF)‐α‐induced apoptosis. MUC1‐transfected SL4 cells injected into ceca (SL4‐M11 cells) or spleens (SL4‐M8 cells) were grown in vivo at the injection sites and as liver metastases. One week later, tumors were collected from the ceca, spleens, and livers and MUC1‐positive cells were obtained. Tumor cells grown (a) in vivo and (b) in vitro were incubated with recombinant mouse TNF‐α and cell viability and proliferation were evaluated using WST‐1 cell proliferation assays. The viability of cells cultured without TNF‐α was regarded as 100%. Data are represented as the mean ± SD. *P < 0.005.