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. 2009 Feb 24;100(4):698–708. doi: 10.1111/j.1349-7006.2009.01102.x

Figure 3.

Figure 3

(A) The upper lanes show examples of IGF2 and H19 expression in germ cell tumors with various methylation statuses of H19 differential methylated region (DMR) (CCCTC‐binding factor binding site 6 [CTCF6]). The lower lanes show examples of SNRPN expression in germ cell tumors with various methylation statuses of SNRPN DMR. (B) Allelic expression analysis of IGF2 and H19 mRNA. Electrophoretic patterns of products of genomic DNA polymerase chain reaction (PCR) or reverse transcritpion‐PCR after ApaI digestion for IGF2, and after RsaI digestion for H19. Biallelic expression of IGF2 was found in Nos. 31, 26 and 44, and monoallelic expression of IGF2 with a faintly stained opposite band was found in Nos. 27, 43 and 45. Three tumors (Nos. 31, 43 and 44) showed monoallelic expression of H19, and one (No. 21) showed biallelic expression of H19. c, control.