Table 3.

Methylation status of H19 and SNRPN DMRs and total and allelic expression of H19, IGF2 and SNRPN

PatientNumber	Pathology	H19 DMR	H19 mRNA	RsaI site	H19 allelic expression	IGF2 mRNA	ApaI site	IGF2 allelic expression	SNRPN DMR	SNRPN mRNA
13	Teratoma	UU	2+	Homo	Not done	2+	Homo	Not done	MM	1+
15	Yolk sac tumor	UU	2+	Homo	Not done	2+	Homo	Not done	UU	2+
21	Yolk sac tumor/embryonal Ca	UU	1+	Hetero	Biallelic	1+	Homo	Not done	UU	2+
24	Yolk sac tumor	UU	2+	Homo	Not done	1+	Homo	Not done	UM	1+
26	Yolk sac tumor	MU	2+	Homo	Not done	2+	Hetero	Biallelic	UU	2+
27	Yolk sac tumor	MU	2+	Homo	Not done	2+	Hetero	Monoallelic †	UU	2+
30	Yolk sac tumor	MU	2+	Homo	Not done	2+	Homo	Not done	UM	2+
31	Yolk sac tumor	MU	2+	Hetero	Monoallelic	2+	Hetero	Biallelic	UM	2+
36	Yolk sac tumor/embryonal Ca	MU	2+	Homo	Not done	2+	Homo	Not done	UM	2+
43	Seminoma	UU	1+	Hetero	Monoallelic	1+	Hetero	Monoallelic †	UU	2+
44	Seminoma	UU	1+	Hetero	Monoallelic	1+	Hetero	Biallelic	UU	2+
45	Seminoma	UU	1+	Homo	Not done	1+	Hetero	Monoallelic †	UU	2+

UU/MM/MU/UM, see the legend for Table 2; Homo, homozygosity; Hetero, heterozygosity; Ca, carcinoma; DMR, differential methylated region; ^†Monoallelic, a faint opposite band is visible.