Figure 6.

 Isolated CD4⁺CD25⁺ T cells from naïve T cells cultured with SKOV3 cell culture supernatant suppress CD4⁺CD25⁻ T cell proliferation. (A) CD4⁺CD25⁺ T cells were isolated by MACS from activated naïve T cells cultured with SKOV3 cell culture supernatant, and FOXP3 expression was detected by flow cytometry before (left) and after (right) sorting. (B) Autologous CD4⁺CD25⁻CD45RA⁻ T cells were cocultured with induced CD25⁺ T cell populations at diffierent ratios (1 : 1, 0.5 : 1, 0.25 : 1, 0 : 1) and the proliferation index (PI) was calculated. The average PI of three independent experiments is shown. Error bars are SD of triplicate samples. Ti, induced CD4+ CD25+ T cells.