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. 2009 Oct 15;101(2):460–467. doi: 10.1111/j.1349-7006.2009.01402.x

Figure 2.

Figure 2

 Effect of branched‐chain amino acid (BCAA) supplementation on the development of foci of cellular alteration (FCA) and on the expression of insulin‐like growth factor (IGF)‐1, IGF‐2 and IGF‐1 receptor (IGF‐1R) mRNAs in the liver of diethylnitrosamine‐treated C57BL/KsJ‐db/db mice. Livers were excised from treated mice supplemented with casein or BCAA for 16 weeks. (a) Paraffin‐embedded liver sections were stained with H&E and the total numbers of FCA were counted. Values are the means ± SD (n = 4). (b–d) Total RNA was isolated from the removed liver and the expression of IGF‐1 (b), IGF‐2 (c), and IGF‐1R (d) genes were examined by quantitative real‐time RT‐PCR. The expression of each gene was normalized to β‐actin expression. Each experiment was done in triplicate. *P < 0.05; **P < 0.01.