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. 2009 Oct 15;101(2):460–467. doi: 10.1111/j.1349-7006.2009.01402.x

Figure 6.

Figure 6

 Effect of branched‐chain amino acid (BCAA) supplementation on the expression of α‐smooth muscle actin (α‐SMA) in diethylnitrosamine‐treated C57BL/KsJ‐db/db mice. (a) Immunohistochemical expression of α‐SMA in the liver of basal diet (CRF‐1)‐fed, casein‐supplemented, or BCAA‐supplemented treated mice. (b) Total protein was extracted from the liver of experimental mice and the expression of α‐SMA protein was examined by Western blot analysis. An antibody to GAPDH served as a loading control. Repeat Western blots gave similar results. The results obtained were quantitated by densitometry and are shown in the right‐hand panels. Values are the means ± SD (n = 5). *P < 0.05; **P < 0.01.