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. 2009 Mar 9;100(6):1051–1061. doi: 10.1111/j.1349-7006.2009.01148.x

Figure 7.

Figure 7

G protein‐coupled receptor (GPR) 30 signaling increased interleukin (IL)‐6 production by endometrial cancer cells. RL95‐2 and KLE were seeded in 24‐well plates at 1 × 106 cells/mL, cultured, and starved. Then the cells were incubated with 10−8 M G1 or 10−8 M 17β‐estradiol (E2) plus U0126 or pertussis toxin (PTX) for 48 h. The supernatants were harvested, centrifuged at 10 000g for 5 min, and the sediments discarded. The supernatants were stored at –80°C until enzyme‐linked immunosorbent assay. (a) IL‐6 secretion in KLE cells. *P < 0.05 versus vehicle; #P < 0.05 versus E2 + U0126 and E2 + PTX; ΔP < 0.05 versus G1 + U0126 and G1 + PTX. (b) IL‐6 secretion in KLE cells. *P < 0.05 versus vehicle; &P < 0.05 versus G1; P < 0.05 versus E2 + U0126 and E2 + PTX; ΔP < 0.05 versus G1 + U0126 and G1 + PTX.