Figure 8.
(A) Effects of anti‐cancer drug on p21 mRNA expression of Mz‐ChA‐2 cells. Cells (1 × 105) were incubated with 400 mM 5‐Fluorouracil (5‐FU) for 48 h with or without 5 μM vorinostat+ 5 ng/mL TGF‐β1. Values are expressed as relative mRNA expression of non‐treated cells. The data were calculated as the means ± SD of three experiments. (B) Effects of vorinostat + TGF‐β1 on 5‐Fluorouracil‐induced growth inhibition of Mz‐ChA‐2 cells. Cells (1 × 105) were incubated with 400 mM 5‐Fluorouracil with or without 5 μM vorinostat + 5 ng/mL TGF‐β1 for 0–72 h. Values are expressed as the rate of growth of non‐treated cells at each point. The data were calculated as the means ± SD of three experiments.