Figure 1.

Three 20‐mer peptides were predicted with a computed algorithm system on the basis of hydrophilicity, probability of surface exposure, flexibility, and antigenic index from the base sequence of the ORF of Gene‐X and thrombospondin (THBS)‐2, and synthesized and purified commercially to over 95%. The identified antigen in this study was located downstream of the registered ORF of THBS‐2. The identified second ORF of THBS‐2 was located from 4506 to 4719 bp, and the prediction of antigenic peptide of THBS‐2 was carried out in the second ORF.