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. 2007 Nov 4;99(1):152–158. doi: 10.1111/j.1349-7006.2007.00651.x

Figure 4.

Figure 4

Macrophage inflammatory protein 1α (MIP‐1α) mRNA expression and protein secretion in MOPC‐31C mouse myeloma cells to which YM529/ONO‐5920 was added with or without geranylgeranyl pyrophosphate (GGPP) or farnesyl pyrophosphate (FPP). (a) MOPC‐31C cells were pretreated with 10 µM GGPP or 10 µM FPP for 4 h then treated with 10 µM YM529/ONO‐5920 for 72 h. Photograph revealed the reverse transcription–polymerase chain reaction analysis of MIP‐1α mRNA expression. These cells receiving YM529/ONO‐5920 and GGPP or FPP were cultured in the presence of 10 µg/mL lipopolysaccharide (LPS) for 1 h. (b) MOPC‐31C cells were pretreated with 10 µM GGPP or 10 µM FPP for 4 h then treated with 10 µM YM529/ONO‐5920 for 72 h. After this treatment, MOPC‐31C cells were incubated for 24 h with 10 µg/mL LPS. Culture supernatant was collected and analyzed for MIP‐1α by enzyme‐linked immunosorbent assay. These results are representative of five independent experiments. *P < 0.01, compared to control.