Figure 6.
Reexpression of estrogen receptor (ER)‐β 0N mRNA by 5‐aza‐2′‐deoxycitidine (5‐aza‐dC) treatment. Methylation status and mRNA expression of exon 0N were assessed by bisulfite sequencing and quantitative reverse transcription–polymerase chain reaction of D0, D3, and D5 with or without 5‐aza‐dC (1.0 µmol/L) treatment. Methylation status showed methylation of 0N promoter CpG sites of each cell line before 5‐aza‐dC treatment.