Figure 5.
Signal transducer and activator of trans‐cription 3 (STAT3) mediates ionizing radiation (IR)‐induced Bcl‐XL accumulation. (a) A549 cells were irradiated. At the indicated times, the levels of Bcl‐XL mRNA were compared via RT‐PCR, using GAPDH as an internal control. (b) The irradiated cells were analyzed for levels of STAT3 protein and its phosphorylation via western blotting. (c) Control and STAT3 siRNAs were introduced into A549 cells. After 24 h of incubation, the cells were irradiated and incubated for an additional 24 h. Levels of the indicated components were compare via western blotting. (d) Cellular invasiveness was compared.