Table 1.
Effect of HBx on cellular transformation in human cells
| Retro virus vector | Cells: | Immortalization † | Colony formation ‡ | Tumor formation§ | ||
|---|---|---|---|---|---|---|
| BJ | TIG3 | BJ‐hTERT | BJ‐hTERT | BJ‐hTERT | ||
| Empty Vector | – | – | + | – | nt | |
| HBx | – | – | + | – | nt | |
| HBx‐D1 | – | – | + | nt | nt | |
| HBx‐D5 | – | – | + | nt | nt | |
| RASv12 | – | – | – | – | nt | |
| RASv12 + HBx | – | – | + | + | 4/8 | |
| RasV12 + HBx‐D1 | – | – | + ¶ | – | 0/8 | |
| RasV12 + HBx‐D5 | – | – | – | – | 0/8 | |
| RasV12 + SV40LT + ST | nt | nt | + | + | 8/8 | |
†
By population doubling (PD) analysis;
‡
‡ by sort agar assay;
§
1 × 106 cells were injected to 8‐week‐old female nude mice.
¶
Growth rate is slower than the cells with those expressing RasV + HBx, and cellular senescence was observed to some extent. All results are by Oishi N, Masutomi K, Murakami et al. (submitted)