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. 2010 Sep 1;101(11):2384–2390. doi: 10.1111/j.1349-7006.2010.01684.x

Figure 2.

Figure 2

 Effect of recombinant mouse interleukin‐17 (rmIL‐17) and conditioned medium on the proliferation and migration of, and tube‐like structure formation by, lymphatic endothelial cells (LEC). (A) After 48 h of culture of LEC with rmIL‐17 (0–500 ng/mL), the number of viable LEC was determined by MTT assay. (B,D) The LEC were seeded in the upper well of a 24‐well trans‐well chamber and the rmIL‐17, LLC medium and LLC/rmIL‐17‐conditioned medium was placed in the lower chamber. After 48 h of incubation the invasive LEC on the outside surface of the upper chambers were visualized by a crystal violet stain (magnification, ×200). (C,E) The LEC were seeded in a 96‐well plate pre‐coated with Matrigel in the presence or absence of rmIL‐17, LLC medium and LLC/rmIL‐17‐conditioned medium, and the formation of capillary‐like structures was assessed. The presence or absence of tube‐like structures was determined after 3 days of incubation (magnification, ×200). In B and C, the results shown are the average of three independent experiments. Data are displayed as the mean ± SD. *P < 0.05, LLC versus rmIL‐17; **P < 0.05, LLC/rmIL‐17 versus LLC.