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Bisulfite sequencing of the estrogen receptor 1 (ESR1) locus in representative patients. In normal mucosal samples, the percentage of methylated references (PMR) were relatively low (6.2 and 22.6) and methylation status shown with bisulfite sequencing was sparse, whereas in adenoma samples, the PMR were high (153.1 and 159.2) and the sequencing revealed that the locus was densely methylated.
