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. 2010 Jul 1;101(10):2145–2155. doi: 10.1111/j.1349-7006.2010.01661.x

Figure 3.

Figure 3

 Characterization of the epithelial cell adhesion molecule (EpCAM)+ and EpCAM− cells in vitro. (A) Sorting of the EpCAM+ and EpCAM− subpopulations from PLC/PRF/5 (upper) and Li‐7 (lower) cells. (B) Proliferation was compared between the EpCAM+ and EpCAM− subpopulations of PLC/PRF/5 and Li‐7. (C) The number of colonies formed in soft agar was determined for the EpCAM+ and EpCAM− subpopulations from PLC/PRF/5 and Li‐7. The average of three independent experiments is shown with SD. (D) Result of a migration assay toward hepatocyte growth factor (HGF). The number of cells that migrated from an upper chamber through a membrane filter was counted using a microscope in five fields selected at random. The average of three independent experiments is shown with SD. * represents P < 0.05.