Figure 8.

 Overexpression and knockdown of epithelial cell adhesion molecule (EpCAM) in cell clones and their characterization in vitro. (A) The proliferation of each clone was compared, as described in Materials and Methods. (B) The number of colonies formed in soft agar was counted as described in Figure 3C. The average of three independent experiments is shown with SD. (C) The migration activity of each cell clone was compared, as described in Figure 3D. The average of three independent experiments is shown with SD. (D) c‐Myc expression in each clone was examined by immunoblotting. Cell lysates were prepared from the indicated cell clones and subjected to immunoblotting using an anti‐c‐Myc antibody. The data represent at least two independent experiments. * represents P < 0.05.