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. 2009 Sep 14;101(1):180–187. doi: 10.1111/j.1349-7006.2009.01366.x

Figure 3.

Figure 3

 Ad5/F35‐shAPE1 effectively knocked down the apurinic/ apyrimidinic endonuclease (APE) 1 expression in a time course. APE1 protein level was assayed by western blotting at 6, 12, 24, 48 and 72 h after 10 MOI Ad5/F35‐shAPE1 transduced to A549 cells. Ad5/F35‐EGFP carrying no shRNA sequence was also infected at the same dose as a negative control. APE1 expression was inhibited at 12 h and significantly knocked down at 48 h after Ad5/F35‐shAPE1 infection and remained at a low level for another 48 h.