Skip to main content
. 2008 Sep 18;99(11):2209–2217. doi: 10.1111/j.1349-7006.2008.00941.x

Figure 8.

Figure 8

Effects of STK15/short hairpin RNA on the activation of apoptotic pathways. (A) Western blotting of untransfected and stably transfected HepG2 cells. Total protein (60 mg) from cell lysates was loaded per lane and blotted with anticleaved caspase‐3 antibodies. Equal loading was confirmed by showing equal β‐actin levels. (B) Caspase‐3 activity of HepG2‐s cells increased by approximately 186%, *P < 0.05. (C) Western blotting of untransfected and stably transfected HepG2 cells. Total protein (60 mg) from cell lysates was loaded per lane and blotted with antibodies against surviving, XIAP, Livin, c‐IAP1, c‐IAP2, Bcl‐2, Bcl‐xl, Bax, Bad and PUMA. Equal loading was confirmed by showing equal β‐actin levels. Lanes: 1, HepG2; 2, HepG2‐U6neo; 3, HepG2‐NS; 4, HepG2‐s.